Warrington Collegiate Student Work Placement

Louis O’Brien, a student from Warrington Collegiate, sitting their BTEC Level 3 National Diploma in Applied Science, applied to do a work placement in the lab of Natasha Savage.

Louis worked with Natasha for a full week during February 2018. They did a variety of experiments and simulations together:

  • Plasmid Preparation. Bulking up plasmids using bacteria, performing a midi plasmid prep.
  • Cell culture. Growing up 3T3 cells from frozen, ready for plasmid transfection.
  • Time Course Imaging. Bacterial colonies, grown from swabs of Louis’s phone, were put in suspension and filmed under a light microscope to observe their proliferation.Polarity Simulations. Louis ran simulations of cells breaking symmetry. By changing reaction strengths Louis worked out the key molecular components.

    Louis doing bacterial work for time course imaging. Growing 3T3 cells from frozen stocks. DNA extracted using midi plasmid prep.

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s