Louis worked with Natasha for a full week during February 2018. They did a variety of experiments and simulations together:
- Plasmid Preparation. Bulking up plasmids using bacteria, performing a midi plasmid prep.
- Cell culture. Growing up 3T3 cells from frozen, ready for plasmid transfection.
- Time Course Imaging. Bacterial colonies, grown from swabs of Louis’s phone, were put in suspension and filmed under a light microscope to observe their proliferation.Polarity Simulations. Louis ran simulations of cells breaking symmetry. By changing reaction strengths Louis worked out the key molecular components.
Louis doing bacterial work for time course imaging. Growing 3T3 cells from frozen stocks. DNA extracted using midi plasmid prep.