How we set about mending damaged knees with stem cells

Anthony Hollander, University of Liverpool

A meniscal tear is one of the most common knee injuries, especially in young, active people. Over a million new cases are diagnosed each year in Europe and the US alone, and it also affects professional sportspeople – footballer Luis Suarez, tennis player Roger Federer and Olympic swimmer Sharon Davies are among the many elite athletes to have suffered a meniscal tear. Unfortunately, there is no effective treatment for this injury, but my team and I believe we are one step closer to providing one. Our “living bandage” uses stem cells and collagen to regrow the damaged meniscal cartilage, which acts as a special kind of shock-absorber.

Knees are rather wonderful things – first class engineering provided through millennia of evolution. Huge forces are channelled through the knees when we stand up, walk or run, amounting to three to five times our body weight in normal daily use. And they must survive these extreme conditions every day while also giving us the flexibility that we enjoy whenever we move. When they work we don’t really notice them. But if we damage them, we suffer incredible pain and lose the freedom to live a normal, active life.

One of the reasons knees work so well is because of the meniscal cartilage, which sits between the bone-ends like stiff cushions. There are two of these menisci in each knee and they are incredibly important for protecting the bones in the joint. If we damage them we are in trouble. However, they can easily tear if we suffer a trauma to the knee and this is a very common type of sporting injury.

Most tears will not heal because there is no blood supply and so no chance for natural healing mechanisms to kick in. The standard way to treat them is an operation to remove the damaged part of the meniscus. This works quite well at first, but, in the long-term, our knees don’t do well with only a part of the meniscus. So, with time, there is wear-and-tear of the bone ends and this often leads to premature osteoarthritis. Once the arthritis sets in, it’s a one-way track to chronic pain and ultimately a joint replacement. Because meniscal tears often happen to young athletic people, this can mean many years of disability from arthritis and great cost to the health service.

MRI of knee with meniscus injury.
Semnic/Shutterstock.com

The long road to finding a cure

My research team began to focus on this problem about 13 years ago. We wanted to deliver living cells with healing properties into the middle of the tear and drive the repair from within. At that time we were studying the biology of a special kind of cell, the mesenchymal stem cell, that is found in small numbers in the bone marrow and which is important for natural healing mechanisms in the body.

But simple injection of the stem cells into the tear was not enough because they didn’t end up in the right place. We had to find a way to hold them close to the two injured surfaces of the tear so that they could migrate into the damaged tissue and release their molecular signals for repair. We eventually found a way to do this by placing them in a membrane made from collagen and inserting the stem cell-collagen combination into the tear. Over a few weeks the stem cells migrated into the surrounding meniscus and then the collagen degrades away, allowing healing across the tear boundary.

We published our findings, filed a patent to protect our discovery and then set about turning the science into medicine, coming up with a product called the Cell Bandage to test in patients. The first five patients have now been treated and although two of them suffered retear, the other three are all doing fine more than three years later. Although repair of the very outer edge of the meniscus is possible without stem cells because it has a blood supply, most of the meniscus is not repairable and all patients would be expected to re-tear within about a year. So the 60% success rate in this first group of patients to be treated with the Cell Bandage is very encouraging indeed and paves the way for much larger trials in the future.

Knees are indeed wonderful things but so too, it seems, are stem cells.

The Conversation

Anthony Hollander, Head of Institute of Integrative Biology, University of Liverpool

This article was originally published on The Conversation. Read the original article.

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Tissue ‘scaffold’ technology could help rebuild large organs

University press release today:

Scientists at the Universities of Liverpool and Bristol have developed a new tissue ‘scaffold’ technology that could one day enable the engineering of large organs.

Researchers have shown that it is possible to combine cells with a special scaffold to produce living tissue in the laboratory. It is hoped this can then be implanted into patients as a way of replacing diseased parts of the body.

Read more here

Listen to Anthony Hollander on BBC One at 6.30 tonight.

You can also read the original article (open access): ‘Artificial membrane-binding proteins stimulate oxygenation of stem cells during engineering of large cartilage tissue’, published in Nature Communications.