Dementia awareness week (15th – 20th May) has all been wrapped up, and in light of the event Dr Jill Madine and her amyloid group (Kieran Hand, Dr Hannah Davies and James Torpey), Prof Jerry Turnbull and Dr Scott Guimond (Institute of Integrative Biology), and Prof Alan Morgan (Institute of Translational Medicine) participated in the Alzheimer’s Research UK North West public engagement event hosted by the University of Salford on Wednesday 17th May 2017. To celebrate the grand opening of the Universities new Dementia hub, scientific researchers from the University of Manchester, MMU, University of Liverpool, University of Salford and Liverpool John Moore’s engaged in an academic event in the morning showcasing what dementia research is taking place at each institution, followed by an afternoon demonstrating their on going efforts to tackle this life changing disease… to the public! A breadth of “hands on” activities were available for all ages, and we also invited Liverpool Life Sciences UTC to get stuck in and showcase their ongoing collaborative projects! Activities ranged from how worms are really changing the way in which we can study dementia (with some brilliant videos) (Morgan group), how a ‘spoonful of sugar’ could help treat dementia (Turnbull group) and all the way to what dementia means to you (Madine group). In this activity, the Madine amyloid group asked individuals or groups if they could write or draw their feelings on dementia, have their photo taken with their work, where the public were delighted with the idea that it’s going to be made into a collage for others that were unable to attend the event to see. There were some truly incredible thoughts on the subject from individuals who had been directly impacted by dementia, and as a group we were incredibly humbled by the positive responses to our ongoing efforts in Alzheimer’s, Parkinson’s and associated disorders. See you next year!
This year our fruitful collaboration with Liverpool life sciences UTC has continued and we would like to take this opportunity to congratulate Laura Hurst on the success of her Year 13 Project.
Laura has been working on our Neuroblastology programme at UTC and designed and carried out an experiment to investigate the neuroprotective effect of lemongrass on brain cells in Alzheimer’s disease. Laura has cultivated SHSY5Y neuronal cells, exposed them to amyloid beta protein (protein involved in neurodegeneration in Alzheimer’s disease) and explored the protective effect of lemongrass on these affected cells. Laura has now finished the project, written an excellent report and presented her findings to her peers and the teachers at the school.
This is her abstract from her report.
This project’s main purpose is to explore the potential neuroprotective effects of lemongrass (Cymbopogon Citratus) and how these effects can be utilised in the treatment of Alzheimer’s disease. The rates of this disease have greatly increased over the past few decades and so the development of new pharmaceuticals is increasingly important to society. To test the hypothesis of lemongrass having neuroprotective effects two well plates were set up with neuroblastoma cultures, one of which had beta amyloid protein (one of the key pathological markers of Alzheimer’s disease) added. Three different solutions of lemongrass essential oil were also added (0.1%,0.5%, and 1%) as well as two control groups containing either F-12 Ham’s nutrient media only or 100% ethanol. The results of the experiment suggested that an increase in lemongrass solution reduced the concentration of cells per mm² but increased the viability of the cells in the amyloid beta protein plate. 0.5% lemongrass solution almost doubled the viability of the neuroblastoma from 37.04% in the media only control group to 68.61%. These results support both the Amyloid hypothesis and the hypothesis established for this project, and so it can be concluded that lemongrass has potential as a treatment to Alzheimer’s disease if further research is carried out.
The school are so impressed they are using her work as a model to show students and teachers alike how science project work should be conducted and reported.
We would all like to congratulate Laura on her fantastic success and wish her luck in her dreams to pursue a career in neuroscience!
In more good news, Dr John Dyer at UTC is involved in the process of arranging an exchange programme to enable students from different schools in Europe to work on extended projects at different sites dependant on their interests. UTC (in collaboration with the University of Liverpool) is hoping to make the neuroblastology project their specialty! So hopefully soon we will be welcoming students from across Europe to learn cell culture techniques and do more exciting experiments.
This was a huge display within the conference venue – amazing photography!
Last week three members of IIB, Dr Hannah Davies, James Torpey and Prof. Jerry Turnbull went to Vienna to find out about the latest research and technological advances in the field of neurodegeneration and dementia at ADPD 2017. This five day conference saw over 3000 clinicians, researchers industry specialists from around the globe discuss recent advances in the field, including reports on the latest drug trails, new avenues for treatment and patient perspectives. This busy meeting gave us the opportunity to catch up with colleagues from around the world, and share the exciting research we are doing here at Liverpool with a huge audience.
The conference venue and an action shot of James presenting his findings at one of the poster sessions
During our stay in Vienna we were treated to welcome reception at Vienna’s beautiful City Hall, we ate traditional Austrian dishes, talked science and enjoyed an impromptu opera performance from one of our colleagues!
Welcome reception in the impressive Vienna city hall
We came away from the conference, tired but full of new ideas and renewed enthusiasm for our projects.
As part of her honours project Mary Roughley visited Mosspits Lane Primary School in Wavertree, Liverpool, during Science week. She has spent an afternoon with each year 6 class and engaged with the pupils on topics such as scales in the universe, the concept and calculation of magnification and the power of using microscopy in biology. As part of her honours project, Mary has planned the session and developed the supporting worksheets and instruction protocols. After a short presentation, the whole class went out onto the playground to collect their own live samples to view under the microscope, the class were then split into three groups to rotate between the three exercises that were organised. The most popular activity was collecting and viewing their samples. The pupils were given magnifying glasses and also had access to the Zeiss stemi labscope to enable them to examine their specimens. They collected insects, worms, leafs, bread crumb, aphids, hairs…They really enjoyed this activity and were fascinated and very excited by what they could see with a microscope: worms digestive tubes, tiny unsuspected hairs on insect legs and “a starry night sky” (salt imaged with transmitted light)!
The pupils also made their own magnifier using water in petri dishes. They learnt how to calculate magnification and used this knowledge to calculate and compare the magnification of a magnifying glass and the magnifier that they made. They realised that their magnifier made with a drop of water was as good as a commercial magnifying glass.
For the third activity, the pupils used the schools computers and an online programme to learn more about scales. The software showed objects of different sizes ranging from galaxies to a proton nucleus. This activity reinforced the idea that microscopes are essentials to biologists, as many things are much too small to be seen with the naked eye. This is what Mary says about her experience: “I received excellent feedback from the pupils. They thoroughly enjoyed the session and some mentioned that they would like to become biologists. They particularly enjoyed using the microscope and collecting their samples and a number of pupils said that the only bad part of the session was packing away! As a proof of the success of the half-day, the teachers had to fight for the children to go out at playtime. They preferred observing their samples under the microscope. I have personally really enjoyed delivering the sessions, I found the experience very rewarding especially when the pupils said they wanted to be biologists! The experience has also made me consider teaching as a career.” This is what the children wrote about the session: “‘I wish the session was longer!!; I liked seeing the intestines in the worm, it was gross but cool!; The bacteria in the pond water was really cool.” It was a very enjoyable experience at all levels: for the children, the teachers, the undergraduate student involved and me, the academic supervisor. Thank you to Mosspits Lane to have worked with us on this project.
Violaine Sée, IIB
On Wednesday 18th January chemistry A-Level students from Range High School visited the Institute for a workshop in the NMR Centre for Structural Biology organised by Dr Jill Madine. This visit has become an annual event which the students enjoy and say enhance their understanding of how NMR can be applied in a research environment. The students learnt about the advantages and disadvantages of mass spec and NMR from Dr Mark Wilkinson and Dr Marie Phelan, carried out chromatography and learnt to prepare and run NMR samples and how to interpret the data. Prior to their visit, as part of a school practical, they have made salicylic acid – a precursor for aspirin. We obtained these samples and collected NMR spectra of their products ready for analysis on the day. This enabled them to establish how successful their synthesis had been and compare their results across the class, with previous years’ students (and to the teacher!).
A range of University of Liverpool postgraduate students and postdocs helped with the day providing practical and theoretical advice, including Dr Hannah Davies, Rudi Grossman, James Torpey and Kieran Hand (pictured above).
Cholangiocarcinoma (CCA) is more prevalent in Southeast Asian countries compared to the western world, where cases of CCA are rare. Opisthorchis viverrini (OV) infection (opisthorchiasis) is the associated cause of this increase of cases in CCA, and rates of high infection with OV correlate with high CCA occurrence. In the Northeastern Isaan region of Thailand, OV-infection is endemic – this region also suffers high levels of CCA. OV may be transmitted by eating raw or undercooked fish in foods such as such as ‘koi pla’. After consumption, the OV parasite resides in the duodenum, the liver and surrounding bile ducts and can live for up to 20 years. Infection is asymptomatic, making CCA difficult to detect until it presents in its terminal stages. For these reasons, OV is classed as a type 1 carcinogen.
As part of our integrated masters final year project and internship, Charlotte and I are observing the sustained immune response towards OV – one of several contributors to the development of CCA. As part of a Newton Fund project funded by the British Council, we have been conducting our research in the Tropical Disease Research Laboratory (TDRL), part of Khon Kaen University. So far, we have enjoyed integrating ourselves into a completely new culture and working in a lab with more independence than we have had before. So far it has been an exciting and rewarding experience. TDRL provides a lot of opportunities for international students, so whilst we are submerged in Thai culture we have also been fortunate to meet people from all across the world. This is in addition to making discoveries along the way in the lab. Knowing that our research will contribute towards efforts to reducing OV-infection and CCA mortalities is very humbling.
TDRL developed the “Lawa Model” several years ago and introduced this to Lawa villages 6 years ago. This model aims to educate people about the dangers of OV and eating potentially contaminated fish. Health volunteers are responsible for screening of patients to detect OV and administering praziquantel, as well as other non-OV health issues. They are also required to examine stool samples and educate locals as part of the free education programme in local schools, or ‘door to door’ educating. The health volunteers are key in maintaining the health and awareness of the local communities, whether it be through interpretive dance, adaptations of popular Thai songs or handing out leaflets.
The Lawa Model education programme in schools is free. Before the model was introduced, an average of 10% of children were OV-positive. Now, most schools around the Lawa Region can boast 0% infection. The Model encourages education about OV as part of the school curriculum by rewarding them with certificates. This is promising for future generations that are much less likely to suffer from CCA.
The Lawa Model adopts the ‘EcoHealth’ approach which means a transdisciplinary approach, where experts from different scientific disciplines such as conchology, ichthyology, parasitology, biochemistry and veterinary medicine that target each stage of OV’s lifecycle. A major failing in previous attempts has been lack of sustained awareness and implementation of control programmes. The Lawa Model has attempted to solve this by encouraging and educating stake holders including local officials, monks, village leaders, schools and the previously mentioned health volunteers who are also responsible for providing regular health checkups for the villagers and checking for OV-infection.
On our visit to Lawa Lake, we saw for ourselves the success of the model, where it was very clear that the majority of people now had a clear understanding of how OV is transmitted. Before the model was introduced six years ago, OV-infection in the human populations in this area was 60%, but this has now fallen to less than 10%. OV-infection in fish has fallen from 70% to <1% and snails infected with OV have dropped to less than 0.2%. As a result of this success, the TDR team are now planning to introduce the Lawa Model into different provinces within Thailand and other neighbouring countries. Charlotte and I recently visited Kalasin, a province nearby to Khon Kaen, which is in very early stages of Lawa Model implementation. It was very eye-opening to see how unaware some people still are in Thailand despite the threat of this disease. However, due to the Lawa Model’s previous success it is likely villages in Kalasin will have the same promising results.
The TDR team from KKU often travels to local Lawa Villages to hold talks about the dangers of OV and explain how it is transmitted. Ultrasound screening is also performed to determine levels of liver complication in patients. Blood samples are also taken from the field and used as part of research about the pathology of OV infection, which precedes CCA. As part of my and Charlotte’s research, we have analysed these blood samples through various immunological assays. Our research will contribute to the current hypothesis that people with a more aggressive immune response are more susceptible to the development of CCA.
The recent ‘National agenda against liver fluke and cancer’ has made clear its aim to remove OV from Thailand in the next 10 years and reduce the number of CCA mortalities. The Lawa Model is a perfect example of how constant attention and support to communities can reduce the infection rate of OV. For this to happen on a national scale, the government must become involved on a permanent level to guarantee replication of the Lawa Model’s success.
After 3 months here, Charlotte and I are continuing to enjoy both the science and the fun that comes with Thai culture. We are fortunate to be part of such a worthwhile project and hope our time here will be beneficial to both ourselves, and the fight against OV and CCA.
Top L-R: Dr Kanin Salao and Eddy processing patient blood samples in the lab; Eddy and Charlotte with Prof Steven Edwards and Dr Helen Wright, who lead the University of Liverpool collaboration with TDRL, and Dr Kanin Salao from TDRL prior to them leaving for Khon Kaen in July; Eddy and Charlotte with other TDRL students paying respects to Prof Banchob Sripa (centre) on “Teachers Day”.
Bottom L-R: Community outreach program in Kalasin; Charlotte carrying out neutrophil isolations in the lab; OV parasite under the microscope.
Guest post by Chris Clarke
On Saturday 1st October Dr Chris Clarke and Dr Dada Pisconti from the Institute of Integrative Biology took part in Meet the Scientists at the World Museum Liverpool. The aim of their stand was to convey to the visitors how muscle is made and repaired. There were two activities set up:
1. Two microscopes displaying samples (from their lab) of muscle progenitor cells fixed at the start (day 0) and end (day 3) of a differentiation experiment where individual cells at the start of the experiment fuse into multinucleated muscle fibers, replicating in vivo muscle formation and regeneration. A laptop was also set up showing a time lapse video of the same process.
2. The Muscle Regeneration game. To show guests the process of muscle regeneration in a more hands-on way, a game was set up here children were asked to repair broken Play-doh ‘muscle fibers’ using Play-doh ‘stem cells’. The aim of the game was to split the stem cell in half, turn one half into a new part of a muscle fiber (ie. A sausage) and fix the broken fiber by ‘fusing’ this new fiber with the broken one. 2-3 sets of broken fibers had to be repaired in this way, with children facing off against siblings/friends/parents to finish in the fastest time possible.
They were also assisted on the day by undergraduate students from the University of Liverpool.
Find out more about future events here.