Guest post by Rosie Maher, IIB PhD student
This summer I welcomed a 16 year old student from The King’s School Chester who took part in the Nuffield Research Placement Scheme. Before starting her placement Charlotte had plans to apply for medicine after finishing her A-levels but was curious about other medical related professions within biomedical science and biochemistry. Charlotte was appointed a position with myself, working in the Centre for Proteome Research within the Institute of Integrative Biology. Her four week project was titled “Identifying Proteins in Saliva to Diagnose Disease” and was a continuation of work that I have completed for my PhD.
During Charlotte’s four week stay she learnt three techniques that are used routinely in our lab; SDS-PAGE, zymography and western blotting. By the end of her placement she had produced some very interesting novel data, complementing the work that I have completed. It was a great experience to teach and supervise Charlotte, especially in an area of medical related science that she hadn’t heard of. I am now looking forward to her presenting this data at the Nuffield Celebration Evening in October.
Louis O’Brien, a student from Warrington Collegiate, sitting their BTEC Level 3 National Diploma in Applied Science, applied to do a work placement in the lab of Natasha Savage.
Louis worked with Natasha for a full week during February 2018. They did a variety of experiments and simulations together:
On Friday 9th March 15 chemistry A-Level students from Range High School visited the Institute for a workshop in the NMR Centre for Structural Biology organised by Dr Jill Madine and Dr Marie Phelan. This visit has been an annual event for the past several years which the students look forward to in order to gain enhanced understanding of NMR to help with their A-level courses and also gain an insight into what goes on in an academic research environment. The students were given lectures on the basic applications of mass spectrometry and NMR from Stephen Moss (School of Physical Sciences) and Dr Marie Phelan. This was the followed by practical workshops where the students carried out chromatography and learnt to prepare and run NMR samples along with how to interpret the data. Prior to their visit, as part of a school practical, they have made salicylic acid – a precursor for aspirin. We obtained these samples and collected NMR spectra of their products ready for analysis on the day. This enabled them to establish how successful their synthesis had been and compare their results across the class, with previous years’ students (and to the teacher!). This final part of the day is always the most exciting for the students where there is no hiding that they actually dropped their sample and scraped it off the desk!
PhD student James Torpey along with internship students Daniel Thomas and Raven Chandramohan helped with the day providing practical and theoretical advice.
In the summer of 2017, two year 12 students from the North West of England visited the Centre for Cell Imaging on their Nuffield research placements.
Their placement involved testing a semi-high-throughput screening method for anti-cancer drugs using cell-migration as their readout. They worked with a Glioblastoma cancer cell line during their time in the facility and shared the following comments about their experiences:
“I believe that this summer placement at the University of Liverpool was the greatest experience of my life, and I will always remember it as the reason I firmly decided that this was the career in which I needed to pursue. I believe that this summer was an eye-opening experience into the real world of science, specifically cell microscopy, and it gave me countless new ideas and theories which I will take away with me into the future, and hopefully begin to research into myself one day. The placement inspired me to want to carry on pursuing science for the rest of my life and fed my ambition to achieve in a new world which I now see with many more possibilities than I had originally perceived.”
“This summer was not the same of all my previous summer, it was amazing and interesting because I spent it in department which I’d like before to be on, I learnt a lot of useful things: using high demand microscope, experiment skills as well as the importance of organisation, planning and time management for each project. This placement gives me the chance to recognise the enjoyable feeling of practical and research world. Also this project allows me to deal comfortably with analysed imaging software which I am never deal with before, these wonderful software will make me think more deeply about the experiment, is not the same of the past (just follow the instructions).”
U87 cells stably expressing a red nuclear marker. Timestamps are HH:MM
Earlier this year sixth formers from Shrewsbury School visited the IIB, hosted by Professor Alan McCarthy.
After listening to lectures around the IIB’s key research themes, the students were given a guided tour.
They gave this report in their newsletter:
“The research facilities at Liverpool are, without exception, world class. We saw their next generation DNA sequencers, capable of reading an entire genome in just a few days; their ‘GeneMill’ for making synthetic DNA; and a ‘laser capture microscope’ that can cut out a single cell from a thin section and capture it for genetic analysis.
“We visited a humming lab filled with mass spectrometers able to detect in real time the chemical fingerprints that differentiate healthy from diseased tissues. We saw the x-ray crystallography and nuclear magnetic resonance imaging suites that are the workhorses in the ongoing effort to model the actual 3D structures of proteins, and in one case we were shown a novel drug being tested against an intricate virtual model of a mutant enzyme.”
X-ray crystallography imaging suite
This year our fruitful collaboration with Liverpool life sciences UTC has continued and we would like to take this opportunity to congratulate Laura Hurst on the success of her Year 13 Project.
Laura has been working on our Neuroblastology programme at UTC and designed and carried out an experiment to investigate the neuroprotective effect of lemongrass on brain cells in Alzheimer’s disease. Laura has cultivated SHSY5Y neuronal cells, exposed them to amyloid beta protein (protein involved in neurodegeneration in Alzheimer’s disease) and explored the protective effect of lemongrass on these affected cells. Laura has now finished the project, written an excellent report and presented her findings to her peers and the teachers at the school.
This is her abstract from her report.
This project’s main purpose is to explore the potential neuroprotective effects of lemongrass (Cymbopogon Citratus) and how these effects can be utilised in the treatment of Alzheimer’s disease. The rates of this disease have greatly increased over the past few decades and so the development of new pharmaceuticals is increasingly important to society. To test the hypothesis of lemongrass having neuroprotective effects two well plates were set up with neuroblastoma cultures, one of which had beta amyloid protein (one of the key pathological markers of Alzheimer’s disease) added. Three different solutions of lemongrass essential oil were also added (0.1%,0.5%, and 1%) as well as two control groups containing either F-12 Ham’s nutrient media only or 100% ethanol. The results of the experiment suggested that an increase in lemongrass solution reduced the concentration of cells per mm² but increased the viability of the cells in the amyloid beta protein plate. 0.5% lemongrass solution almost doubled the viability of the neuroblastoma from 37.04% in the media only control group to 68.61%. These results support both the Amyloid hypothesis and the hypothesis established for this project, and so it can be concluded that lemongrass has potential as a treatment to Alzheimer’s disease if further research is carried out.
The school are so impressed they are using her work as a model to show students and teachers alike how science project work should be conducted and reported.
We would all like to congratulate Laura on her fantastic success and wish her luck in her dreams to pursue a career in neuroscience!
In more good news, Dr John Dyer at UTC is involved in the process of arranging an exchange programme to enable students from different schools in Europe to work on extended projects at different sites dependant on their interests. UTC (in collaboration with the University of Liverpool) is hoping to make the neuroblastology project their specialty! So hopefully soon we will be welcoming students from across Europe to learn cell culture techniques and do more exciting experiments.