A-level students at the School of Medicine at West Lancashire College were inspired by talks from three biochemists Dr Jill Madine, Dr Nigel Jones and Professor Sonia Rocha during November and December 2019. Students learnt about the research carried out within our research groups relating to diseases and their underpinning mechanisms and the career paths each of us followed to reach our current positions. The students were some of the most attentive audience members we have ever spoken to asking questions that wouldn’t be out of place at large International Scientific Conferences. We hope to welcome some of the students for summer work experience placements in IIB.
46 year 12 pupils and 4 teachers from Shrewsbury School visited IIB on Wednesday 8th January to discover the amazing technology and facilities we have within the institute. The day organised by Dr Jill Madine began with an unmissable opportunity to promote UG courses at Liverpool from Dr Andy Bates, School of Life Sciences. The pupils then learnt about a wide range of applications using the available technologies from Dr Marie Phelan, Dr Linda D’Amore, Dr Gareth Wright and Professor Pat Eyers. In the afternoon the students got to see the facilities up close and ask questions during tours of Barkla X-Ray laboratory of Biophysics, NMR Centre for Structural Biology and Centre for Genomic Research from PhD students Liam McCormick and Kangsa Amporndanai.
Guest post by Rosie Maher, IIB PhD student
This summer I welcomed a 16 year old student from The King’s School Chester who took part in the Nuffield Research Placement Scheme. Before starting her placement Charlotte had plans to apply for medicine after finishing her A-levels but was curious about other medical related professions within biomedical science and biochemistry. Charlotte was appointed a position with myself, working in the Centre for Proteome Research within the Institute of Integrative Biology. Her four week project was titled “Identifying Proteins in Saliva to Diagnose Disease” and was a continuation of work that I have completed for my PhD.
During Charlotte’s four week stay she learnt three techniques that are used routinely in our lab; SDS-PAGE, zymography and western blotting. By the end of her placement she had produced some very interesting novel data, complementing the work that I have completed. It was a great experience to teach and supervise Charlotte, especially in an area of medical related science that she hadn’t heard of. I am now looking forward to her presenting this data at the Nuffield Celebration Evening in October.
Louis worked with Natasha for a full week during February 2018. They did a variety of experiments and simulations together:
- Plasmid Preparation. Bulking up plasmids using bacteria, performing a midi plasmid prep.
- Cell culture. Growing up 3T3 cells from frozen, ready for plasmid transfection.
- Time Course Imaging. Bacterial colonies, grown from swabs of Louis’s phone, were put in suspension and filmed under a light microscope to observe their proliferation.Polarity Simulations. Louis ran simulations of cells breaking symmetry. By changing reaction strengths Louis worked out the key molecular components.
Louis doing bacterial work for time course imaging. Growing 3T3 cells from frozen stocks. DNA extracted using midi plasmid prep.
On Friday 9th March 15 chemistry A-Level students from Range High School visited the Institute for a workshop in the NMR Centre for Structural Biology organised by Dr Jill Madine and Dr Marie Phelan. This visit has been an annual event for the past several years which the students look forward to in order to gain enhanced understanding of NMR to help with their A-level courses and also gain an insight into what goes on in an academic research environment. The students were given lectures on the basic applications of mass spectrometry and NMR from Stephen Moss (School of Physical Sciences) and Dr Marie Phelan. This was the followed by practical workshops where the students carried out chromatography and learnt to prepare and run NMR samples along with how to interpret the data. Prior to their visit, as part of a school practical, they have made salicylic acid – a precursor for aspirin. We obtained these samples and collected NMR spectra of their products ready for analysis on the day. This enabled them to establish how successful their synthesis had been and compare their results across the class, with previous years’ students (and to the teacher!). This final part of the day is always the most exciting for the students where there is no hiding that they actually dropped their sample and scraped it off the desk!
PhD student James Torpey along with internship students Daniel Thomas and Raven Chandramohan helped with the day providing practical and theoretical advice.
This journal is founded by year 12 students from Liverpool Life Sciences UTC with support from Senior Editors from Liverpool and Wigan UTC, University of Sheffield and Dr Hannah Davies, Institute of Integrative Biology, University of Liverpool. Hannah’s involvement began a couple of years ago working with the students to design and run projects incorporating cell culture as a research tool supported by a Biochemical Society Outreach grant (link to previous posts). This journal provides an excellent way for students to engage with other young scientists around the world and develop their skills in written scientific communication and networking. Through reporting their research findings they will develop important skills that will be invaluable in their future careers. The journal has received a lot of attention and positive feedback. We praise all of the contributors and editors for their hard work and hope that the BSJ will continue to grow in the coming months and years. Please visit the first edition of the journal here.
Their placement involved testing a semi-high-throughput screening method for anti-cancer drugs using cell-migration as their readout. They worked with a Glioblastoma cancer cell line during their time in the facility and shared the following comments about their experiences:
“I believe that this summer placement at the University of Liverpool was the greatest experience of my life, and I will always remember it as the reason I firmly decided that this was the career in which I needed to pursue. I believe that this summer was an eye-opening experience into the real world of science, specifically cell microscopy, and it gave me countless new ideas and theories which I will take away with me into the future, and hopefully begin to research into myself one day. The placement inspired me to want to carry on pursuing science for the rest of my life and fed my ambition to achieve in a new world which I now see with many more possibilities than I had originally perceived.”
“This summer was not the same of all my previous summer, it was amazing and interesting because I spent it in department which I’d like before to be on, I learnt a lot of useful things: using high demand microscope, experiment skills as well as the importance of organisation, planning and time management for each project. This placement gives me the chance to recognise the enjoyable feeling of practical and research world. Also this project allows me to deal comfortably with analysed imaging software which I am never deal with before, these wonderful software will make me think more deeply about the experiment, is not the same of the past (just follow the instructions).”